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Quantification and Identification of Bioactive Eugenol in Myristica fragrans Seed Using Validated High Performance Thin Layer Chromatography Technique.

Quantification and Identification of Bioactive Eugenol in Myristica fragrans Seed Using Validated High Performance Thin Layer Chromatography Technique.

A sensitive, selective, precise and stability indicating high-performance thin layer chromatographic

 method (HPTLC) was developed and validated for analysis of eugenol from methanolic extract of Myristica fragrans seed. The chromatography study was performed on aluminium foil-backed silica gel 60 F-254 HPTLC plates using toluene-ethyl acetate-formic acid (2:5:0.2, v/v/v) as the mobile phase. The developed analytical method were found to be linear in the concentration range of 100-1000 ng band-1 with regression value closer to unity (r2=0.998). The developed system was found to give compact spots for eugenol (Rf 0.55) with the limit of detection and limit of quantification (21 and 63 ng band-1) respectively. Further the study showed recovery of eugenol in the range of 99.3-99.8% with the precision value ≤ 1.85% and ≤ 1.71%.

Quantification and Identification of Bioactive Eugenol in Myristica fragrans Seed Using Validated High Performance Thin Layer Chromatography Technique. Performed by Mr Mohamad Taleuzzaman under the supervision of Dr Sadaf Jamal Gilani.