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  2. Dr. Imran Hussain
Dr. Imran Hussain

Dr. Imran Hussain

Assistant professor of School of Life Science, Glocal University
PhD (Biotechnology) University of Texas at Arlington, USA

EDUCATION

Qualification

Year

University

Ph.D.

2016

University of Texas at Arlington, USA

MTech(AgriculturalBiotech)

2012

IIT Kharagpur,India

MSc(Bot., Zool.&Chemistry)

2008

DDU Gorakhpur University, India


Qualified Exam
  • NET (Life Science) 2009, CSIR-UGC
  • GATE (Life Science;AIR:137) 2010, IIT
  • GRE(International Exam)
  • TOEFL (International Exam)

TEACHINGS (2010-15) AND COURSES TAUGHT
  1.  Lectured in classes of the following courses at University of Texas at Arlington, USA
  • Freshman level general chemistry courses: Lectured to undergraduate student
  • Biochemistry: Lectured to senior undergraduate student
  • Chemistry to non-science majors: Lectured to undergraduate student
  1. Mentored graduate and undergraduate students towards successful completion of their research projectsat University of Texas at Arlington, USA
HONORS, AWARDS AND RESEARCH ACHIEVEMENTS
    • Received 2nd place in poster presentation at Green Center Retreat (June 14-15, 2018) in Pottsboro, TX organized by UT Southwestern Medical Center, USA
    • Received 3rd place in platform presentation at Texas Forum for Reproductive Sciences 22nd annual meeting (April 21-22, 2018) in Houston, TX, USA
    • Received graduate teaching assistantship (2012-16) at University of Texas at Arlington, USA
    • Received STEM doctoral fellowship (2012-16) at University of Texas at Arlington, USA
    • Received GATE fellowship (2010-12) from Ministry of HRD, govt. of India
    • Received DBT merit scholarship (2008-10) from Ministry of HRD, govt. of India 
    • Qualified national-levelMSc Biotechnology entrance exam (2008) conducted by Jawaharlal Nehru University, India
    • Google Scholar Citations: 1225; h-index: 14; i10-index:14
RESEARCH TECHNICAL SKILLS
  • Molecular biological and biochemical techniques: Extensivemolecular cloning (includes TA cloning and sub-cloning), luciferase assay, RT-PCR, RT-qPCR, western blotting, siRNA- or lentiviral-mediated knockdown experiment, chromatin immunoprecipitation experiment, Co-immunoprecipitation, isolation of RNA, protein, genomic DNA, and plasmid DNA, gel electrophoresis (agarose and SDS-PAGE), Silver staining, 2D gel electrophoresis, triglycerides and free fatty acid measurement on mouse plasma and liver tissue, detailed steps of protein expression and purification, downstream processing of extracellular enzymes, thin layer chromatography, gel filtration chromatography and BCA protein estimation assay.
  • Mouse work:Extensive handling of mice giving intraperitoneal and subcutaneous injection of estrogen, progesterone, and RU486 with regards to preterm birth studies and sacrificed mice to isolate endometrium and other organs. Gave intraperitoneal injection of glucose and insulin to diabetic mouse model and performed IPGTT and IPITT studies.
  • Animal cell culture: Extensive cell culturing of normal cells, cancer cells, stable cell lines and transfected cells.
  • Drug development: Screened small molecules in apoptosis and hormonal assays (cell culture and mice model) and various cytotoxic assays to screen potential drugs.
  • Others: Xenograft implantation in nude mice to perform antisense therapy trials,immunohistochemistry,immuncytochemistry, cytotoxicity assays (such asDNA cleavage,MTT, caspase, cytochrome-c and TUNEL), plant tissue culture, basic microbiological techniques, FACS analysis, ELISA, cell synchronization for cell cycle study, analysis of polyphenols by HPLC and UV-Vis spectrophotometry.
  • Bioinformatics and other computer programs: Blast, fasta, gene and protein sequence analysis on pubmed, primers and antisense sequencesdesigning, eukaryotic linear motif resource for analysis of functional domains in proteins, NEB cutter, multalin, ORF finder, end note, chemdraw,imageJ, Graph pad prism, Target scan program for miRNA target prediction, Transcription factor binding analysis software such as tfbind, matinspector, and PROMO, adobe photoshop and microsoft office (word, excel and power point).
SHORT-TERM RESEARCH PROJECTS AND TRAININGS       
  • One year dissertation work on“Hexavalent chromium reduction by a green microalga Scenedesmus obliquus: A prospective for chromium detoxification” at Dept. of Agricultural & Food Engineering, IIT Kharagpur, under the supervision of Dr.Nirupama Mallick. 
  • Four months dissertation work on “Studies on chlorophyll a synthesis in cyanobacteria by laser induced fluorescence and UV-Vis spectrophotometer grown in the presence of heavy metals” at Centre for Biotechnology, University of Allahabad, under the supervision of Dr.Shanthy Sundaram. 
  • Two months training on“Biobleaching of wood pulp using laccase producing bacteria” at Institute of Genomics & Integrative Biology, CSIR (New Delhi), under the guidance of Dr. Rita Kumar.
JOURNAL PUBLICATIONS 
    1. Hussain I, Bhan A, Ansari KI, Deb P, Bobzean SA, Perrotti LI, Mandal SS. Bisphenol-A induces expression of HOXC6, an estrogen-regulated homeobox-containing gene associated with breast cancer,BiochimBiophys Acta 2015;1849(6):697-708
    2. HussainI, Deb P, Chini A, Obaid M, BhanA, Ansari KI, Mishra BP, Bobzean SA, Udden SMN, Alluri PG, Das HK, Brothers RM, Perrotti LI, Mandal SS. HOXA5 expression is elevated in breast cancer and is transcriptionally regulated by estradiolFront Genet 2020;11.
    3. Ansari KI*, Hussain I*, Shrestha B,Kasiri S, Mandal SS. HOXC6 is transcriptionally regulated via coordination of MLL histone methylase and estrogen receptor in an estrogen environment,J Mol Biol 2011;411(2):334-49. *contributed equally.
    4. Hussain I,Kwak YT, Mendelson CR. Declining levels of corepressors CtBP1/2, CNOT1, and GATAD2B during late gestation serve to impair progesterone receptor function and the initiation of labor, J Biol Chem 2020, under revision.
    5. Chen C-C, Montalbano AP, Hussain I, Lee W-R, Mendelson CR.The transcriptional repressor GATAD2B mediates progesterone receptor suppression of myometrial contractile gene expression,J Biol Chem 2017;292(30):12560-76.
    6. Deb P,Bhan A, Hussain I, Ansari KI, Bobzean SA, Saha D, Perrotti LI, Mandal SS.Endocrine disrupting chemical, bisphenol-A, induces breast cancer associated gene HOXB9 expression in vitro and in vivo,FASEB J 2016;1(30):1053.
    7. Mootha VV, Hussain I, Cunnusamy K, Graham E, Gong X, Neelam S, Xing C, Kittler R, Petroll WM. TCF4 triplet repeat expansion and nuclear RNA foci in Fuchs' endothelial corneal dystrophy,Invest Ophthalmol Vis Sci 2015;56(3):2003-11
    8. Xing C, Gong X, Hussain I, Tan D, Aung T, Mehta J, Vithana E, Mootha VV. Transethnic replication of association of CTG18.1 repeat expansion of TCF4 gene with fuchs corneal dystrophy in Chinese implies common causal variant,Invest Ophthalmol Vis Sci 2014;55(11):7073-80. 
    9. Ansari KI, Hussain I, Kasiri S, Mandal SS. HOXC10 is overexpressed in breast cancer and transcriptionally regulated by estrogen via involvement of histone methylases MLL3 and MLL4,J Mol Endocrinol 2012;48(1):61-75.
    10. Ansari KI, Shrestha B, Hussain I, Kasiri S, Mandal SS. Histone methylases MLL1 and MLL3 coordinate with estrogen receptors in estrogen-mediated HOXB9 expression, Biochemistry 2011;50(17):3517-27.
    11. Ansari KI, Hussain I, Das H, Mandal SS. Overexpression of human histone methylase MLL1 upon exposure to a food contaminant mycotoxin, deoxynivalenol,FEBS J 2009;276 (12):3299-307.
    12. Ansari KI, Kasiri S, Hussain I, Mandal SS. Mixed lineage leukemia histone methylases play critical roles in estrogen-mediated regulation of HOXC13, FEBS J 2009;276(24):7400-11.
    13. Shrestha B, Ansari KI, Bhan A, Kasiri S, Hussain I, Mandal SS. Homeodomain-containing protein HOXB9 regulates expression of growth and angiogenic factors, facilitates tumor growth in vitro and is overexpressed in breast cancer tissue, FEBS J 2012;279(19):3715-26.
    14. Ansari KI, Kasiri S, Hussain I, Bobzean SA, Perrotti LI, Mandal SS. MLL histone methylases regulate expression of HDLR-SR-B1 in presence of estrogen and control plasma cholesterol in vivo, Mol Endocrinol 2013;27(1):92-105.
    15. Kasiri S, Ansari KI, Hussain I, Mandal SS. Antisense oligonucleotide-mediated knockdown of HOXC13 affects cell growth and induces apoptosis in tumor cells and over expression of HOXC13 induces 3D-colony formation,RSC Adv2013;3(10):3260-69.
    16. Bhan A, Hussain I, Ansari KI, Kasiri S, Bashyal A, Mandal SS. Antisense transcript long noncoding RNA (lncRNA) HOTAIR is transcriptionally induced by estradiol,J Mol Biol 2013;425(19):3707-22. 
    17. BhanA,Hussain I, Ansari KI, Bobzean SA, Perrotti LI, Mandal SS. Histone methyltransferase EZH2 is transcriptionally induced by estradiol as well as estrogenic endocrine disruptors bisphenol-A and diethylstilbestrol,J Mol Biol 2014;426(20):3426-41. 
    18. BhanA,Hussain I, Ansari KI, Bobzean SA, Perrotti LI, Mandal SS. Histone methyltransferase EZH2 is transcriptionally induced by estradiol as well as estrogenic endocrine disruptors bisphenol-A and diethylstilbestrol,J Mol Biol 2014;426(20):3426-41. 
    19. Bhan A, Hussain I, Ansari KI, Bobzean SA, Perrotti LI, Mandal SS. Bisphenol-A and diethylstilbestrol exposure induces the expression of breast cancer associated long noncoding RNA HOTAIR in vitro and in vivo,J Steroid Biochem Mol Biol 2014;141:160-70. 
    20. Deb P,Bhan A, Hussain I,  Ansari KI, Bobzean SA, Pandita TK, Perrotti LI, Mandal SS.Endocrine disrupting chemical, bisphenol-A, induces breast cancer associated gene HOXB9 expression in vitro and in vivo,Gene 2016;590(2):234-43.
BOOK CHAPTER PUBLICATION
  1. Mandal SS, Ansari KI, Hussain I, Bhan A. Impacts of deoxynivalenolon human cells: oxidative stress, misregulation of histone methyl-transferases, HOX genes, and epigenetics; Mycotoxins: Properties, Applications and Hazards 2011, Page No. 45-84. (Book edited by Nova Science Publishers, New York).
PLATFORM PRESENTATIONS IN CONFERENCES 
  1. Hussain I, Chen CC, Montalbano AP, Truong P, and Mendelson CR. C-terminal binding protein 1 (CtBP1) and GATAD2B serve as novel mediators of progesterone/PR suppression of proinflammatory and contractile genes in the pregnant myometrium, TFRS 22nd Annual Meeting, Houston, April 21-22, 2016.
  2. Hussain I, Chen CC, Montalbano AP, Truong P, and Mendelson CR. C-terminal binding protein 1 (CtBP1) and GATAD2B serve as novel mediators of progesterone/PR suppression of proinflammatory and contractile genes in the pregnant myometrium, The Endocrine Society’s 98th Annual Meeting and Expo, Boston, April 1-4, 2016.
  3. Hussain I, Ansari KI, Kasiri S, Shrestha B, and Mandal SS. HOXC6 is transcriptionally regulated via coordination of MLL histone methylase and estrogen receptor under estrogen environment, Regional Meeting of the ACS, New Orleans, December 1, 2012.
  4. Hussain I, Ansari KI, Shrestha B, Kasiri S, and Mandal SS. MLL histone methylases in estrogen-mediated regulation of HOXC6, ACS meeting in miniature, UT Dallas, April 17, 2010.
  5. Hussain I, Ansari KI, Kasiri S, Shrestha B, and Mandal SS. Estrogen-mediated regulation of HOXC6, ACES, UT Arlington,March 25, 2012.
  6. Hussain I, and Mendelson CR. CNOT1 serves as a mediator of progesterone/PR suppression of contractile gene expression in the pregnant mouse myometrium, TFRS 23rd Annual Meeting, Houston, April 27-28, 2017.
POSTER PRESENTATIONS IN CONFERENCES 
  1. Hussain I, Chen CC, Montalbano AP, Truong P, and Mendelson CR. C-terminal binding protein 1 (CtBP1) and GATAD2B serve as novel mediators of progesterone/PR suppression of proinflammatory and contractile genes in the pregnant myometrium, Green Center Retreat, Pottsboro-TX, June 14-15, 2016.
  2. Hussain I, Gong X, and Mootha VV. Triplet Repeat Primed PCR Assay to Genotype the CTG18.1 Trinucleotide Repeat Polymorphism in TCF4.ARVO Annual Meeting, Orlando, May 4-8, 2014.
  3. Mandal SS, Ansari KI, Kasiri S, Hussain I, and Shrestha B. MLL histone methyl-transferases in gene regulation, epigenetics and steroid signaling, DFW estrogen receptor focus group quarterly meeting, UNT Health Science Center, July 2, 2010.
  4. Mandal SS, Ansari KI, Kasiri S, Hussain I, and Shrestha B. MLL histone methyl-transferases in steroid hormone-mediated regulation of HOX genes. Gordon conference on chromatin structure and function, Bryant University in Smithfield RI United States, July 25-30, 2010.
  5. Mandal SS, Ansari KI, Kasiri S, and Hussain I. Mixed lineage leukemia histone methylases in estrogen signaling and cardiovascular disease, Regional meeting of the ACS, New Orleans, December 1, 2010.
  6. Bhan A, Ansari KI, Shrestha B, Hussain I, Kasiri S, and Mandal SS. Histone methylase MLL1 and MLL3 coordinate with estrogen receptor in estrogen-mediated HOXB9 expression, ACES, UT Arlington,March,2012.
  7. Ansari KI,Hussain I, Kasiri S, and Mandal SS. Estrogen-mediated regulation of HOXC6: Implication in breast and prostate cancer, DFW estrogen receptor focus group quarterly meeting, UNT Health Science Center, July 2, 2011.
  8. Mandal SS, Ansari KI, and Hussain I. Targeting mixed lineage leukemia histone methylases for novel cancer therapy. Regional meeting of the ACS, El Paso, November 4, 2009. 
  9. Mandal SS, Ansari KI, Kasiri S, Hussain I, and Shrestha B. Mixed lineage leukemias in steroid hormone signaling, epigenetics and human disease, Chemistry in the 21st century: challenges and opportunities, University of Kalyani, May 28-29, 2010.
  10. Mandal SS, Ansari KI, Hussain I, Kasiri S, and Shrestha B. Mixed lineage leukemia histone methylases in estrogen-mediated HOX gene expression and disease. Cold spring harbor-asia symposium on epigenetics, chromatin and transcription, Suzhou, China, May 17-21, 2010.
  11. Mandal SS, Ansari KI, Kasiri S, and Hussain I. MLL histone methylases in estrogen-mediated regulation of HOX genes involved in hair follicle development and leukemia, ASBMB meeting on Chromatin and transcription, Anaheim, CA, April 24-27, 2010.
  12. Kasiri S, Ansari KI, Hussain I, Shrestha B, and Mandal SS. Mixed lineage leukemia histone methylases play critical roles in estrogen-mediated regulation of HOXC13, ACS meeting in miniature, UT Dallas, April 17, 2010.
  13. Shrestha B, Ansari KI, Hussain I, Kasiri S, and Mandal SS. Histone methylases MLL1 and MLL3 coordinate with estrogen receptors in estrogen-mediated HOXB9 expression, DFW estrogen receptor focus group quarterly meeting, UT Arlington, February 26, 2011.
  14. Kasiri S, Ansari KI, Hussain I, and Mandal SS. Mixed lineage leukemia histone methylases play critical roles in estrogen-mediated regulation of HOXC12, ACES, UT Arlington,March 25, 2009.
  15. Ansari KI, Grant JD, Kasiri S, Woldemariam GA, Shrestha B, Hussain I, and Mandal SS. Metallo-salens as potential anti-tumor agent. College of science research day, UT Arlington, October 3, 2008.
  16. Bhan A, Ansari KI, Shrestha B, Hussain I, Kasiri S, and Mandal SS. Histone methylases MLL1 and MLL3 coordinate with estrogen receptors in estrogen-mediated HOXB9 expression,DFW estrogen receptor focus group quarterly meeting, UNT Health Science Center, July 2, 2011.
  17. Ansari KI, Mishra BM, Shrestha B, Kasiri S, Hussain I, Reddy BM, and Mandal SS. MLL histone methyl-transferases in gene regulation, cardiovascular disease and cancer, College of science research day, UT Arlington, October 3, 2008.
  18. Kasiri S, Ansari KI, Hussain I, and Mandal SS. Manganese (III)-salensinduce tumor selective apoptosis in human cells, Regional meeting of the ACS, El Paso, November 4, 2009.
  19. Ansari KI, Kasiri S, Hussain I, and Mandal SS. Mixed lineage leukemia histone methylases regulate human HDLR-SR-B1 and blood cholesterol, Regional meeting of the ACS, El Paso, November 4, 2009.
  20. Ansari KI,Hussain I, Kasiri S, and Mandal SS. Estrogen-mediated regulation of HOXC6: Implication in breast and prostate cancer, DFW estrogen receptor focus group quarterly meeting, UT Arlington, February 26, 2011.
REVIEWER OF JOURNAL 
  • Molecular and Cellular Endocrinology
  • BMC Molecular Biology
PROFESSIONAL MEMBERSHIPS
  • Endocrine Society
  • The Association for Research in Vision and Ophthalmology;Member ID Number: 247575
  • American Association for the Advancement of Science; Member ID Number: 20300359
  • American Association of Immunologists; Member ID Number: 1128146
PERSONAL QUALITIES AND OTHERS
    • Hard working, willingness to learn, good analytical skill, ability to work well in a team, excellent teaching ability, good manuscript writing and reviewing ability.
  • Wrote a grant in 2014 entitled “Molecular and Genetic Basis of Fuchs' Endothelial Corneal Dystrophy” and submitted for CTM translational pilot award program at UT Southwestern Medical Center, Dallas, TX.
  • Wrote one research paper entitled “Declining levels of corepressors CtBP1/2, CNOT1, and GATAD2B during late gestation serve to impair progesterone receptor function and the initiation of labor” and submitted to Journal of Biological Chemistry for publication.
  • Registered national bone marrow donor with donor ID # 1443-8557-2.